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2 edition of Mechanism of S-adenosylmethionine transport in Saccharomyces cerevisiae. found in the catalog.

Mechanism of S-adenosylmethionine transport in Saccharomyces cerevisiae.

James E. Talmadge

Mechanism of S-adenosylmethionine transport in Saccharomyces cerevisiae.

by James E. Talmadge

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Published .
Written in English


The Physical Object
Paginationviii, 35 l.
Number of Pages35
ID Numbers
Open LibraryOL16736794M

  S-adenosyl-L-methionine is an important bioactive molecule participating in a number of biochemical reactions including the transmethylation and transsulphuration reactions of proteins and the biosynthesis of aliphatic polyamines. Strategies of metabolic engineering were used to alter the metabolic flux for enhancing the production of S-adenosyl-L-methionine Cited by: 5. S-Adenosyl-l-methionine (SAMe) is a derivative of methionine and a cofactor for multiple synthetic pathways, particularly as a methyl group donor. It is produced naturally in the body, mainly by the liver, and is manufactured synthetically in supplement form. (See also Overview of Dietary.

Nitrogen-regulated Ubiquitination of the Gap1 Permease ofSaccharomyces cerevisiae. Gap2 Is the Only General Amino Acid Permease and Gap4 Is an S -Adenosylmethionine (SAM) Transporter Required for SAM-Induced Morphogenesis. 21 December | mSphere, Vol. 1, No. 6 Molecular Biology of the Cell is published by the American Society for.   () Transport of sulfonium compounds: characterization of the S-adenosylmethionine and S-methylmethionine permeases from the yeast Saccharomyces cerevisiae. J Biol Chem – OpenUrl Abstract / FREE Full Text.

These sequence features have been used to recognize genes encoding MCs (in humans, called SLC25) in the genomes of various organisms. As a result, 53 members in humans, 35 in Saccharomyces cerevisiae and 58 in Arabidopsis thaliana [5,6] have been detected. Most MC genes encode proteins consisting almost exclusively of the three sequence repeats Cited by: 3.   S-adenosylmethionine is a source of diverse chemical groups used in biosynthesis and modification of virtually every class of biomolecules. The most notable reaction requiring S-adenosylmethionine, transfer of methyl group, is performed by a large class of enzymes, S-adenosylmethionine-dependent methyltransferases, which have been the focus of Cited by:


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Mechanism of S-adenosylmethionine transport in Saccharomyces cerevisiae by James E. Talmadge Download PDF EPUB FB2

Saccharomyces cerevisiae SAM, a mutant yeast strain with high S-adenosylmethionine synthetase activity (approximately 60 U/mL), was screened by flow cytometer following the method described in our previous study.

The slant medium consisted of 10 g/L yeast extract, 20 g/L peptone, 20 g/L glucose and 20 g/L by: 5. A gene for ethionine resistance isolated from the yeast Saccharomyces cerevisiae DKD‐5D‐H conferred on the yeast cells resistance to seleno‐ L ‐methionine and capability to produce S‐adenosyl‐ L ‐methionine in the cells.

An enzymatic study of the L ‐methionine synthetic pathway of L ‐methionine proto‐ and auxotrophs and in dried yeast cells with or without the gene. Abstract. Yall, Irving (University of Arizona, Tucson, Ariz.). Biosynthesis of S-adenosylmethionine by Saccharomyces cerevisiae.I.

Adenine and methionine requirements. Bacteriol. – —Both a parent strain (SC) and an adenineless mutant strain (SC) of Saccharomyces cerevisiae accumulated S-adenosylmethionine (AM) in the presence Author: Irving Yall. Saccharomyces cerevisiae has been used in several industrial processes such as for the production of S-adenosyl-l-methionine (SAM) [1, 2].SAM is synthesized from l-methionine and ATP in S.

cerevisiae, and acts as a biological methyl group donor involved in many metabolic reactions such as transmethylation of metabolite has been widely used in Cited by: 3. In Saccharomyces cerevisiae, the MET25 gene encodes O-acetylhomoserine sulfhydrylase. Synthesis of this enzyme is repressed by the presence of S-adenosylmethionine (AdoMet) in the growth medium.

We identified cis elements required for MET25 expression by analyzing small deletions in the MET25 promoter region. Biosynthesis of Methionine in Saccharomyces cerevisiae KINETICS AND MECHANISM OF REACTION OF S-ADENOSYLMETHIONINE: HOMOCYSTEINE METHYLTRANSFERASE” STANLEY K.

SHAPIRO, ALDONA ALMENAS, AND JOHN F. THOMSON I”yorn the Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois 60&O. S-adenosyl-l-methionine (AdoMet) is synthesized by transfer of the adenosyl moiety of ATP to the sulfur atom of methionine.

This reaction is catalysed by AdoMet synthetase. In all eukaryotic organisms studied so far, multiple forms of AdoMet synthetases have been reported and from their recent study, it appears that AdoMet synthetase is an exceptionally Cited by: In the biosynthesis of S-adenosylmethionine (SAM) in baker’s yeast (Saccharomyces cerevisiae), ATP functions as both a precursor and a driving force.

However, few published reports have dealt with the control of ATP concentration using genetic design. In this study we have adopted a new ATP regulation strategy in yeast for enhancing SAM biosynthesis, including altering NADH Cited by: 2. The gene for ethionine resistance was isolated, and its phenotypic characteristics were investigated.

The cells transformed with this gene showed strong resistance to DL-ethionine, and S-adenosylmethionine (SAM) was remarkably accumulated within the cells. Judging from the restriction map of this gene, it suggests that the gene is not the gene SAM1 but by: 9.

Biochimica et Biophysica Acta, () Elsevier Scientific Publishing Company, Amsterdam - Printed in The Netherlands BBA KINETIC PROPERTIES OF S-ADENOSYLMETHIONINE:d2'-STEROL METHYLTRANSFERASE ENZYMES) IN MITOCHONDRIAL STRUCTURES OF SACCHAROMYCES CEREVISIAE R.

BAILEY, E. Cited by: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

GO ID GO Aspect Molecular Function Description Enables the transfer of S-adenosylmethionine from one side of a membrane to the other. S-adenosylmethionine is S-(5'-adenosyl)-L-methionine, an important intermediate in one-carbon metabolism. In Saccharomyces cerevisiae, the methylation of homocysteine with X-adenosylmethionine as methyl donor is the only enzy- matic synthesis of methionine that has been reported in cell-free systems, although this does not preclude the existence of other pathways.

For insight into the mechanism and significance of. «hide 10 20 30 40 50 mdilkrgnes dkftkietes ttipndsdrs gslirrmkds fkqsnlhvip 60 70 80 90 edlenseqte qekiqwklas qpyqkvlsqr hltmiaiggt lgtglfiglg yslasgpaal ligfllvgts mfcvvqsaae lscqfpvsgs yathvsrfid esvgftvatn yalawlisfp seligcalti sywnqtvnpa vwvaifyvfi mvlnlfgvrg faetefalsi ikviaififi iigivliagg gpnstgyiga.

In this work, we further introduced SAM2 gene and ERC1, a frame-shift mutant of YHRw, to maximize the production of S-adenosylmethionine in S. cerevisiae sake K6. The recombinant strain accumulated SAM about half of its dry cell weight. KW - Metabolic Engineering. KW - S-adenosyl-L-methionine.

KW - Saccharomyces cerevisiae sake. Effect of a potent methylation inhibitor oxidized adenosine (Adox), and a universal methyl group donor S-adenosyl-L-methionine (AdoMet) on trehalose metabolism was studied in.

The medium and fed-batch fermentation by Saccharomyces cerevisiae S-W55 were optimized. The unpolished rice from aging paddy was used as major nutrient source to reduce the raw material cost of SAM fermentation.

The unpolished rice saccharificate (URS) and yeast extract were employed as carbon source and nitrogen source, respectively. The dosages of URS and Author: Wen Jun Wang. S-adenosylmethionine (also known as SAMe) is a manmade form of a chemical that occurs naturally in the body.

SAMe has been used in alternative medicine as a likely effective aid in reducing the 5/5. Regulation of S-Adenosylmethionine Levels in Saccharomyces cerevisiae Article in Journal of Biological Chemistry (44) November with 22 Reads How we measure 'reads'.

cerevisiae sake K6 was the firstly isolated industrial strain to overproduce S-adenosyl-L-methionine (SAM). Although the strain has advantages over other strains, such as GRAS (generally recognized as safe) property, the S. cerevisiae K6 has not been further developed with DNA recombinant technology due to the lack of a proper genetic by: 8.

Fig. 1. Selenomethionine toxicity and S-adenosylmethionine synthesis.(A) Diagram of methionine and AdoMet metabolic pathways in S. cerevisiae.(B) Selenomethionine resistance of sam1Δ and sam2Δ strains bearing the indicated mutations were serially diluted and spotted on SD-methionine minimal media containing 60 μM AdoMet and increasing concentrations of.

In the yeast Saccharomyces cerevisiae, As(V) uptake takes place similarly through the phosphate transporters (Wysocki and Tamas, ), whereas As(III) enters the cell principally through the aquaglyceroporin Fps1, a bidirectional channel that normally functions to transport glycerol (Wysocki et al., ; Maciaszczyk-Dziubinska et al., )./deliver/fulltext///refhtml?itemId=/content/book//ref01&mimeType=html&fmt=ahah.

1. Abbe, E. Some remarks on the.